2.1 – Good Work Practices/Procedures For Handling Laboratory Chemicals

2.1.1 – General Guidelines

Carefully read the label before using a chemical. The manufacturer or supplier’s Safety data sheet (SDS) will provide special handling information. Be aware of the potential hazards existing in the laboratory and the appropriate safety precautions. Know the location and proper use of emergency equipment, the appropriate procedures for responding to emergencies, and the proper methods for storage, transport and disposal of chemicals within the facility.

Do not work alone in the laboratory. If you must work alone or in the evening, let someone else know and have them periodically check on you.

Label all secondary chemical containers with appropriate identification and hazard information (see Section 1.5.2.2, Container Labeling).

Use only those chemicals for which you have the appropriate exposure controls (such as a chemical fume hood) and administrative programs/procedures (training, restricted access, etc.). Always use adequate ventilation with chemicals. Operations using large quantities (500 milliliters) of volatile substances with workplace standards at or below 50 ppm should be performed in a chemical fume hood.

Use hazardous chemicals and all laboratory equipment only as directed or for their intended purpose.

Inspect equipment or apparatus for damage before adding a hazardous chemical. Do not use damaged equipment.

Inspect personal protective apparel and equipment for integrity or proper functioning before use.

Malfunctioning laboratory equipment (i.e. hoods) should be labeled or tagged “out of service” so that others will not inadvertently use it before repairs are made. Report all malfunctioning laboratory safety equipment to the Director of EHS or Facility Manager wnelligan@wesleyan.edu or ckeeler01@wesleyan.edu.

Handle and store laboratory glassware with care. Do not use damaged glassware. Use extra care with Dewar flasks and other evacuated glass apparatus; shield or wrap them to contain chemicals or fragments should implosion occur.

Do not dispense more of a hazardous chemical than is needed for immediate use.

2.1.2 – Personal Hygiene
  • Wear chemical goggles or other appropriate eye protection at all times.
  • Wear gloves when handling chemicals. A selection Guide is available in the Sciences Stockroom Remove gloves before leaving any lab or before touching any common environmental surfaces.
  • Remove contaminated clothing and gloves before leaving laboratory.
  • Avoid direct contact with any chemical. Keep chemicals off your hands, face and clothing, including shoes. Never smell, inhale or taste a chemical. Wash thoroughly with soap and water after handling any chemical.
  • Smoking, drinking, eating and the application of cosmetics is forbidden in laboratories where hazardous chemicals are used.
  • Never pipette by mouth. Use a pipette bulb or other mechanical pipette-filling device.
2.1.3 – Housekeeping
  • Keep floors free of clutter, clean and dry.
  • Keep all aisles, hallways, and stairs clear of all chemicals. Stairways and hallways should not be used as storage areas.
  • Keep all work areas, and especially work benches, clear of clutter and obstructions.
    All working surfaces should be cleaned regularly, including the laboratory sink and fume hood.
  • Access to emergency equipment, utility controls, showers, eyewashes, and exits should never be blocked.
  • Wastes should be kept in the appropriate containers and labeled properly. Full instructions located at your Satellite Accumulation Site.
  • All chemical bottles or containers must be properly labeled.